| Autor: | Galeno, Hector, Mora, Judith, Villagra, Eliecer, Fernandez, Jorge, Hernandez, Jury, Mertz, Gregory J., Ramirez, Eugenio |
| Revista: | Emerging Infectious Diseases |
| Año: | 2002 |
| Número: | 7 |
| Paginas: | 657-661 |
| Volumen: | 8 |
| Abstract: | We isolated Andes virus (formal name: Andes virus ANDV, a species in the genus Hantavirus), from serum of an asymptomatic 10-year-old Chilean boy who died 6 days later of hantavirus pulmonary syndrome (HPS). The serum was obtained 12 days after his grandmother died from HPS and 2 days before he became febrile. No hantavirus immunoglobulin (Ig) G or IgM antibodies were detected in the serum sample. After three blind passages, ANDV antigens were detected in Vero E6 cells by immunofluorescence assay and enzyme-linked immunosorbent assay, and ANDV RNA was detected by reverse transcription-polymerase chain reaction. A fragment of the virus genome showed 96.2% nucleotide identity with that of prototype ANDV. To our knowledge, this is the first isolation of any agent of hemorrhagic fever with HPS from a human and the first such isolation of hantavirus before symptoms of that syndrome or HPS began. |
| Idioma: | Ingles |
| Base de Datos: | PubMed |
| Ver Más: | http://www.ncbi.nlm.nih.gov/pubmed/12095430 |