| Autor: | Quezada-Aguiluz, Mario, Opazo-Capurro, Andrés, Lincopan, Nilton, Esposito, Fernanda, Fuga, Bruna, Mella-Montecino, Sergio, Riedel, Gisela, Lima, Celia A., Bello-Toledo, Helia, Cifuentes, Marcela, Silva-Ojeda, Francisco, Barrera, Boris, Hormazábal, Juan C., González-Rocha, Gerardo |
| Revista: | Antibiotics (Basel, Switzerland) |
| Año: | 2022 |
| Número: | 9 |
| Paginas: | 1207 |
| Volumen: | 11 |
| Abstract: | Carbapenem-resistant Enterobacterales (CRE) is a critical public health problem in South America, where the prevalence of NDM metallo-betalactamases has increased substantially in recent years. In this study, we used whole genome sequencing to characterize a multidrug-resistant (MDR) Klebsiella pneumoniae (UCO-361 strain) clinical isolate from a teaching hospital in Chile. Using long-read (Nanopore) and short-read (Illumina) sequence data, we identified a novel un-typeable megaplasmid (314,976 kb, pNDM-1_UCO-361) carrying the blaNDM-1 carbapenem resistance gene within a Tn3000 transposon. Strikingly, conjugal transfer of pNDM-1_UCO-361 plasmid only occurs at low temperatures with a high frequency of 4.3 × 10-6 transconjugants/receptors at 27 °C. UCO-361 belonged to the ST1588 clone, previously identified in Latin America, and harbored aminoglycoside, extended-spectrum ?-lactamases (ESBLs), carbapenem, and quinolone-resistance determinants. These findings suggest that blaNDM-1-bearing megaplasmids can be adapted to carriage by some K. pneumoniae lineages, whereas its conjugation at low temperatures could contribute to rapid dissemination at the human-environmental interface. |
| Idioma: | en |
| Base de Datos: | PubMed |
| Ver Más: | https://doi.org/10.3390/antibiotics11091207 |