| Autor: | Quezada-Aguiluz, Mario, Aguayo-Reyes, Alejandro, Carrasco, Cinthia, Mejías, Daniela, Saavedra, Pamela, Mella-Montecinos, Sergio, Opazo-Capurro, Andrés, Bello-Toledo, Helia, Munita, José M., Hormazábal, Juan C., González-Rocha, Gerardo |
| Revista: | Antibiotics (Basel, Switzerland) |
| Año: | 2022 |
| Número: | 8 |
| Paginas: | 1000 |
| Volumen: | 11 |
| Abstract: | Macrolides, lincosamides, and type B streptogramins (MLSB) are important therapeutic options to treat methicillin-resistant Staphylococcus aureus (MRSA) infections, however, resistance to these antibiotics has been emerging. In Chile, data on the MLSB resistance phenotypes are scarce in both community-(CA) and hospital-acquired (HA) MRSA isolates. Antimicrobial susceptibility to MLSB was determined for sixty-eight non-repetitive isolates of each HA-(32) and CA-MRSA (36). Detection of SCCmec elements, ermA, ermB, ermC, and msrA genes was performed by PCR. The predominant clones were SCCmec I-ST5 (HA-MRSA) and type IVc-ST8 (CA-MRSA). Most of the HA-MRSA isolates (97%) showed resistance to clindamycin, erythromycin, azithromycin, and clarithromycin. Among CA-MRSA isolates, 28% were resistant to erythromycin, azithromycin, and 25% to clarithromycin. All isolates were susceptible to linezolid, vancomycin, daptomycin and trimethoprim/sulfamethoxazole, and over 97% to rifampicin. The ermA gene was amplified in 88% of HA-MRSA and 17% of CA-MRSA isolates (p <, 0.001). The ermC gene was detected in 6% of HA-SARM and none of CA-SARM isolates, whereas the msrA gene was only amplified in 22% of CA-MRSA (p <, 0.005). Our results demonstrate the prevalence of the cMLSB resistance phenotype in all HA-MRSA isolates in Chile, with the ermA being the predominant gene identified among these isolates. |
| Idioma: | en |
| Base de Datos: | PubMed |
| Ver Más: | https://doi.org/10.3390/antibiotics11081000 |